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The serum concentration of cholesterol was estimated using a reagent kit (Reactivos Spinreact) according to the method described by Deeg and Ziegenohrm [ 26 ]. The serum level of triglycerides was determined using a reagent kit (Reactivos Spinreact) according to the method described by Fossati and Prencipe [ 27 ]. The serum level of high-density lipoprotein cholesterol (HDL-C) was analyzed according to the method reported by Norbert [ 28 ]. Serum very-low-density lipoprotein cholesterol (vLDL-C) and low-density lipoprotein cholesterol (LDL-C) levels were calculated following the equation of Friedewald et al. [ 29 ]:

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